Reversed enzyme activity DNA interrogation test: a new method for mutation detection

Michelle Mandrekar; Ken Lewis; Donna Leippe; Ryan Olson; John W. Shultz

doi:10.1117/12.380502

22 March 2000 Reversed enzyme activity DNA interrogation test: a new method for mutation detection

Michelle Mandrekar, Ken Lewis, Donna Leippe, Ryan Olson, John W. Shultz

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380502
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The Reversed Enzyme Activity DNA Interrogation Test (READIT^TM) System provides a new, rapid method for genotype determination. The system can be used for the analysis of single nucleotide polymorphisms as well as insertions, deletions and chromosomal rearrangements. The READIT^TM System is based upon driving various enzymatic reactions in the reverse direction from the direction they are normally considered. The method is based on a pyrophosphorylation reaction that is the reverse of DNA polymerization. Interrogation probes annealed to DNA fragments with which they have a 3- mismatch are not a substrate for the reaction and do not generate significant signals compared with probes that match the interrogation sequence perfectly. By interrogation of specific DNA sequences with carefully designed probes, mutant genes can be identified with speed and precision.

Citation Download Citation

Michelle Mandrekar, Ken Lewis, Donna Leippe, Ryan Olson, and John W. Shultz "Reversed enzyme activity DNA interrogation test: a new method for mutation detection", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380502

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