UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: an electron paramagnetic resonance (EPR) study

Rachel Lubart; R. Lavie; Harry Friedmann; M. Sinyakov; Asher Shainberg; Haim Breitbart; Nili Grossman

doi:10.1117/12.405915

3 November 2000 UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: an electron paramagnetic resonance (EPR) study

Rachel Lubart, R. Lavie, Harry Friedmann, M. Sinyakov, Asher Shainberg, Haim Breitbart, Nili Grossman

Author Affiliations +

Proceedings Volume 4159, Effects of Low-Power Light on Biological Systems V; (2000) https://doi.org/10.1117/12.405915
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The stimulatory effect of low energy light (LEL) has been attributed to irradiation-induced ROS formation. In the present study we demonstrate that irradiating various cell cultures such as fibroblasts, cardiac and sperm cells with UVA or various light sources in the visible range results in singlet oxygen and OH radical formation. These radicals were monitored by using the EPR technique. We believe that the light induced ROS could mediate previously documented effects of LEL on these cells.

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Rachel Lubart, R. Lavie, Harry Friedmann, M. Sinyakov, Asher Shainberg, Haim Breitbart, and Nili Grossman "UVA and visible light-induced reactive oxygen species (ROS) formation in cell cultures: an electron paramagnetic resonance (EPR) study", Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); https://doi.org/10.1117/12.405915

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