Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells

Gennady G. Levin; Theodor V. Bulygin; Eugene Kalinin; Gennady N. Vishnyakov; Irene V. Goryainova

doi:10.1117/12.447441

2 November 2001 Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells

Gennady G. Levin, Theodor V. Bulygin, Eugene Kalinin, Gennady N. Vishnyakov, Irene V. Goryainova

Author Affiliations +

Proceedings Volume 4431, Photon Migration, Optical Coherence Tomography, and Microscopy; (2001) https://doi.org/10.1117/12.447441
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The authors have proposed and tested the new type of the microscope with superimposed interference and fluorescence images of the living cell. The fluorescence image gives the information about the distribution of drugs in different parts of the cell. Interference microscopy technique allows implementing the quantitative analysis of a living transparent cell, for example, to measure dry cell weight and cell density spatial distribution. Interference microscopy makes possibility the observation cell vital activity at dynamics. Computer-aided processing of interferograms using phase shift technique, allows carrying out continuous (over 12 hours and more) monitoring of living cells quantitative parameters with time interval between measurements less than one minute. RMS error for dry weight of non-living fixed test object using this microscope is about 1%.

Citation Download Citation

Gennady G. Levin, Theodor V. Bulygin, Eugene Kalinin, Gennady N. Vishnyakov, and Irene V. Goryainova "Application of fluorescence-interference microscope for monitoring of dry weight dynamics and drug distribution within living cells", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); https://doi.org/10.1117/12.447441

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available