Imaging collagen orientation using polarization-modulated second harmonic generation

Patrick Christian Stoller; Peter M. Celliers; Karen M. Reiser; Alexander M. Rubenchik

doi:10.1117/12.470690

17 June 2002 Imaging collagen orientation using polarization-modulated second harmonic generation

Patrick Christian Stoller, Peter M. Celliers, Karen M. Reiser, Alexander M. Rubenchik

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470690
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We use polarization-modulated second harmonic generation to image fiber orientation in collagen tissues, with an axial resolution of about 10 micrometers and a transverse resolution of up to 1 micrometers . A linearly polarized ultra-short pulse (200 fs) Ti:Sapphire laser beam is modulated using an electro-optic modulator and quarter-wave plate combination and focused onto a translation stage mounted sample using a microscope objective. The generated second harmonic light is collected using a photomultiplier tube and demodulated using phase sensitive detection to obtain signal intensity and fiber orientation information. In order to obtain second harmonic generation images of different types of collagen organization, we analyze several different tissues, including rat-tail tendon, mouse aorta, mouse fibrotic liver, and porcine skin. We can use our technique to image fibrotic tissue in histological sections of damaged liver and to identify burned tissue in porcine skin to a depth of a few hundred microns. Polarization-modulated second harmonic generation potentially could be a useful clinical technique for diagnosing collagen related disease or damage, especially in the skin.

Citation Download Citation

Patrick Christian Stoller, Peter M. Celliers, Karen M. Reiser, and Alexander M. Rubenchik "Imaging collagen orientation using polarization-modulated second harmonic generation", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470690

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