Multiphoton microscopy of antigen presenting cells in experimental cancer therapies

Simon C. Watkins; Glenn D. Papworth; Lori A. Spencer; Adriana T. Larregina; Holger Hackstein

doi:10.1117/12.470684

17 June 2002 Multiphoton microscopy of antigen presenting cells in experimental cancer therapies

Simon C. Watkins, Glenn D. Papworth, Lori A. Spencer, Adriana T. Larregina, Holger Hackstein

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470684
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The absence of effective conventional therapy for most cancer patients justifies the application of novel, experimental approaches. One alternative to conventional cytotoxic agents is a more defined molecular approach for cancer immune treatment; promotion of the immune system specifically to target and eliminate tumor cells on the basis of expression of tumor-associated antigens (TAA). TAA could be presented to T-cells by professional antigen-presenting cells (APC) that generate a more efficient and effective anti-tumor immune response. In fact, it has been well documented that dendritic cells, the most immunologically potent APC, are capable of recognizing, processing and presenting TAA, in turn initiating a specific antitumor immune response. Results from several laboratories and clinical trials suggested significant but still limited efficacy of TAA-pulsed dendritic cells administered to tumor-bearing hosts. Following such delivery, it is fundamentally necessary to dynamically assess cell abundance within the microenvironment of the tumor in the presence of the appropriate therapeutic agent. Multiphoton microscopy was used to assess the trafficking of pulsed dendritic cells and other APC in skin, lymph nodes and brain of several animal tumor models, following different routes of administration.

Citation Download Citation

Simon C. Watkins, Glenn D. Papworth, Lori A. Spencer, Adriana T. Larregina, and Holger Hackstein "Multiphoton microscopy of antigen presenting cells in experimental cancer therapies", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470684

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