High-sensitivity detection and monitoring of microcirculation using cutaneous and catheter probes for Doppler optical coherence tomography

Victor X.D. Yang; M. L. Gordon; B. Qi; E. Seng Yue; S. Tang; Stuart K. Bisland; J. Pekar; S. Lo; Norman E. Marcon M.D.; B. Wilson; Alex Vitkin

doi:10.1117/12.503214

23 July 2003 High-sensitivity detection and monitoring of microcirculation using cutaneous and catheter probes for Doppler optical coherence tomography

Victor X.D. Yang, M. L. Gordon, B. Qi, E. Seng Yue, S. Tang, Stuart K. Bisland, J. Pekar, S. Lo, Norman E. Marcon M.D., B. Wilson, Alex Vitkin

Author Affiliations +

Proceedings Volume 4965, Optical Diagnostics and Sensing in Biomedicine III; (2003) https://doi.org/10.1117/12.503214
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Background: Currently clinical Doppler ultrasound cannot detect microvascular blood flow and it is difficult to provide depth discrimination using laser Doppler flowmetry. Doppler optical coherence tomography (DOCT) is a novel technique for noninvasive subsurface imaging of microcirculation and tissue structure. Aims: To design handheld and catheter-based DOCT probes for clinical cutaneous and endoscopic imaging. To develop signal processing techniques for real-time detection and quantification of microvascular blood flow. Methods: A DOCT system, with interchangeable cutaneous and catheter probes, was developed. The axial spatial resolution was 10 μm, and the velocity resolution was 20 μm/s, using a 1300 nm broadband infrared light. The system achieved real-time imaging with frame rates up to 32 Hz at 512 x 256 pixels per frame. We used the system to detect microcirculation in human skin and rat esophagus, and to monitor microvascular responses to photodynamic therapy (PDT) in a rat tumor model. Results: We present experimental results from in vivo DOCT imaging of microcirculation in human skin arterio-venous malformations (AVM), normal rat esophagus, and a rat gliosarcoma PDT model. In the PDT model, we followed microvascular responses to PDT and observed differences in the microcirculation during and after therapy, which can have important implications for PDT dosimetry and treatment optimization. Conclusions: To our knowledge, this is the first demonstration of endoscopic catheter-based DOCT detection of microcirculation in vivo. In addition, AVM can be detected using handheld cutaneous DOCT probes under clinical settings. DOCT may serve as a real-time monitoring tool for PDT dosimetry, especially for vascular targeting photosensitizers.

Citation Download Citation

Victor X.D. Yang, M. L. Gordon, B. Qi, E. Seng Yue, S. Tang, Stuart K. Bisland, J. Pekar, S. Lo, Norman E. Marcon M.D., B. Wilson, and Alex Vitkin "High-sensitivity detection and monitoring of microcirculation using cutaneous and catheter probes for Doppler optical coherence tomography", Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); https://doi.org/10.1117/12.503214

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available