Use of acceptor fluorescence for determining FRET lifetimes

Michael K Hughes; Simon M. Ameer-Beg; Marion Peter; Tony Ng

doi:10.1117/12.500641

9 October 2003 Use of acceptor fluorescence for determining FRET lifetimes

Michael K Hughes, Simon M. Ameer-Beg, Marion Peter, Tony Ng

Author Affiliations +

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500641
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

A technique is presented to utilize time-resolved fluorescence from acceptors in donor-acceptro Forster resonance energy transfer (FRET) fluorophore pairs to improve the efficiency and accuracy of FRET lifetime determinations. This technique was applied experimentally and the results presented. This technique could be useful in fluorescence-liftime imaging systems which can measure more than one wavelength channel simultaneously.

Citation Download Citation

Michael K Hughes, Simon M. Ameer-Beg, Marion Peter, and Tony Ng "Use of acceptor fluorescence for determining FRET lifetimes", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500641

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