Investigation of the hybridization process in DNA microarrays by fluorescence lifetime imaging

Daniela Comelli; Cosimo D'Andrea; Paola Taroni; Gianluca Valentini; Rinaldo Cubeddu; Clarissa Consolandi; Gianluca De Bellis; Luigi Rossi Bernardi

doi:10.1117/12.501069

8 October 2003 Investigation of the hybridization process in DNA microarrays by fluorescence lifetime imaging

Daniela Comelli, Cosimo D'Andrea, Paola Taroni, Gianluca Valentini, Rinaldo Cubeddu, Clarissa Consolandi, Gianluca De Bellis, Luigi Rossi Bernardi

Author Affiliations +

Proceedings Volume 5141, Diagnostic Optical Spectroscopy in Biomedicine II; (2003) https://doi.org/10.1117/12.501069
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

cDNA-microarrays for expression profiling rely on the assumption that the fluorescence signal is linearly proportional to the concentration of the markers. Yet, interactions amongst different markers and with the substrate might affect the chromophore quantum yield and lead to an erroneous reading. To indirectly evaluate quantum yield changes and their relevance for DNA-microarray reading, we studied the effect on fluorescence lifetime by using a system for fluorescence lifetime imaging. Quenching of the fluorescence emission were observed in the tight environment represented by a DNA microarray due to concentration and to interactions with the processing chemicals present on the substrate.

Citation Download Citation

Daniela Comelli, Cosimo D'Andrea, Paola Taroni, Gianluca Valentini, Rinaldo Cubeddu, Clarissa Consolandi, Gianluca De Bellis, and Luigi Rossi Bernardi "Investigation of the hybridization process in DNA microarrays by fluorescence lifetime imaging", Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); https://doi.org/10.1117/12.501069

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available