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13 July 2004 Ho:YAG laser in reshaping tracheal cartilage: a pilot investigation using ex vivo porcine and rabbit cartilage
Anthony Lam, Dmitry E. Protsenko, Nicholas Carbone, Chao Li, Ryan Jackson, Brian Jet-Fei Wong M.D.
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Proceedings Volume 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV; (2004) https://doi.org/10.1117/12.543542
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States
Abstract
Stenotic, collapsed, and flow-restricted tracheal airways may result from blunt trauma, chronic infection, and the prolonged endotracheal intubation. This pilot investigation characterizes the degree of shape change produced by Ho:YAG laser (λ=2.12 μm) irradiation of rabbit and pig trachea tissue as a function of laser dosimetry and application protocol. Force displacement curves were generated using fresh lagomorph and porcine tracheal cartilage rings secured in a modified single beam cantilever geometry. These specimens were then irradiated for varying amounts of time and power with the objective of straightening these curved specimens. The degree of shape change was documented photographically. Force and surface temperature were monitored. Confocal microscopy was then used in combination a vital staine (“live-dead assay”) to determine the level of viability of straightened cartilage for selected exposure time-power pairs. Laser Cartilage Reshaping of the trachea may provide a new method to treat severe tracheal injuries without the need for classic open surgical techniques. This pilot investigation is the first step toward demonstrating the feasibility of this technique. Long-term, the design of stents combined with laser irradiation may provide a means to alter tracheal shape.
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Anthony Lam, Dmitry E. Protsenko, Nicholas Carbone, Chao Li, Ryan Jackson, and Brian Jet-Fei Wong M.D. "Ho:YAG laser in reshaping tracheal cartilage: a pilot investigation using ex vivo porcine and rabbit cartilage", Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); https://doi.org/10.1117/12.543542
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KEYWORDS
Cartilage
Laser irradiation
Tissues
Confocal microscopy
Injuries
Laser tissue interaction
Photography
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