Paper
1 July 2004 Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells
Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Randolph D. Glickman, Benjamin A. Rockwell
Author Affiliations +
Abstract
Human retinal pigment epithelial (RPE) cells (hTERT-RPE1) were used to detect photo-oxidation products generated from chronic NIR (810 nm) laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H2DCF-DA and its analog CM-H2DCF-DA. Fluorescence was detected in cells exposed to mode-locked (76 MHz, ~160 femtoseconds) but not CW laser exposure. Detection of photo-oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H2DCF-DA dye proved a more sensitive indicator of oxidation than H2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures (using the most sensitive fluorescent dye conditions) when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode-locked exposure.
© (2004) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Randolph D. Glickman, and Benjamin A. Rockwell "Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.529355
Lens.org Logo
CITATIONS
Cited by 2 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Oxidation

Mode locking

Luminescence

Continuous wave operation

Near infrared

Laser damage threshold

Microscopes

Back to Top