Reducing and shaping the fluorescence detection volume for single bio-molecules analysis: the assets of nanophotonic structures

Herve Rigneault; Emilien Etienne; Pierre-Francois Lenne

doi:10.1117/12.545087

15 September 2004 Reducing and shaping the fluorescence detection volume for single bio-molecules analysis: the assets of nanophotonic structures

Herve Rigneault, Emilien Etienne, Pierre-Francois Lenne

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Proceedings Volume 5450, Photonic Crystal Materials and Nanostructures; (2004) https://doi.org/10.1117/12.545087
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

We review and discuss recent concepts and methods used to detect single fluorescent molecules with sub-wavelength resolution for biological analysis. We start with a brief review of the far field techniques that are able to define an observation volume, which is of the order or smaller than the optical diffraction limit. In a second part, we discuss how photonics structures can be used to shape both the excitation and the emitted optical fields, leading to a better signal to noise ratio and an enhancement of the temporal and spatial resolutions. We show that this 'nano-optical field engineering' can be associated with single molecule detection techniques such as Fluorescence Correlation Spectroscopy. In this framework, we illustrate the potentialities of planar photonic structures for the study of confined molecular diffusion in cells and bacteria.

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Herve Rigneault, Emilien Etienne, and Pierre-Francois Lenne "Reducing and shaping the fluorescence detection volume for single bio-molecules analysis: the assets of nanophotonic structures", Proc. SPIE 5450, Photonic Crystal Materials and Nanostructures, (15 September 2004); https://doi.org/10.1117/12.545087

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KEYWORDS

Diffusion

Fluorescence correlation spectroscopy

Mirrors

Molecules

Luminescence

Confocal microscopy

Near field optics

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