Observations on the forward-scattered speckle patterns from healthy human fibroblasts and melanoma cells

Sean J. Kirkpatrick; Leif Vigeland; Donald D Duncan

doi:10.1117/12.590422

29 March 2005 Observations on the forward-scattered speckle patterns from healthy human fibroblasts and melanoma cells

Sean J. Kirkpatrick, Leif Vigeland, Donald D Duncan

Proceedings Volume 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II; (2005) https://doi.org/10.1117/12.590422
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Confluent monolayers of Malme-3M (ATCC HTB-64) cells, which is a melanoma cell line, and Malme-3 (ATCC HTB-102) cells, which are normal skin fibroblast cells, were cultured and plated onto glass coverslips. The two lines were isolated from the same patient, providing a tumor and normal counterpart for comparative studies. The forward-scattered objective speckle patterns were observed using a HeNe lasers that emitted at 543 and 633 nm. First and second-order speckle statistics from the two cell types were compared and contrasted. Of particular interest were the mean and maximum speckle size for a given observation geometry, degree of polarization, contrast, and the intensity probability distribution function. The speckle patterns from dry, densely-packed single layer latex sphere samples of various sizes were also investigated for comparison. The results indicate that the far-forward speckle patterns from these confluent monolayers can be used to discriminate between the cell types and can be used to derive specific morphological parameters of the cells.

Citation Download Citation

Sean J. Kirkpatrick, Leif Vigeland, and Donald D Duncan "Observations on the forward-scattered speckle patterns from healthy human fibroblasts and melanoma cells", Proc. SPIE 5696, Complex Dynamics and Fluctuations in Biomedical Photonics II, (29 March 2005); https://doi.org/10.1117/12.590422

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