Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Rachel J. Errington; Paul J. Smith; Simon Ameer-Beg; Boris Vojnovic

doi:10.1117/12.590201

28 April 2005 Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, Boris Vojnovic

Author Affiliations +

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.590201
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We report on the development of a stroboscopic excitation technique using a self-pulsing laser, and show that it is a feasible method for obtaining fluorescence lifetime information from a biochip format. The self-pulsing lasers described here are versatile devices which have been used for one photon excitation measurements to determine the lifetime of cyanine 5 in water and ethanol. The same devices have been used to develop a technique whereby the emphasis for time-resolution of a lifetime measurement can be transferred to the excitation source from the detector and processing electronics by virtue of the multiple-pulse, variable frequency nature of the laser output.

Citation Download Citation

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, and Boris Vojnovic "Time-resolved fluorescence measurements using self-pulsing 650-nm laser diodes", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.590201

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