The affect of low-coherent light on microbial colony forming ability and morphology of some gram-positive and gram-negative bacteria

Denis Ed. Popov; Elena S. Tuchina; Julia A. Chernova; Dmitry Podshibyakin; Dmitry V. Rudik; Maria Samsonova; Igor Gromov; Valery V. Tuchin

doi:10.1117/12.634773

22 June 2005 The affect of low-coherent light on microbial colony forming ability and morphology of some gram-positive and gram-negative bacteria

Denis Ed. Popov, Elena S. Tuchina, Julia A. Chernova, Dmitry Podshibyakin, Dmitry V. Rudik, Maria Samsonova, Igor Gromov, Valery V. Tuchin

Author Affiliations +

Proceedings Volume 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI; (2005) https://doi.org/10.1117/12.634773
Event: Saratov, Russia, 2004, Saratov, Russian Federation

Abstract

Gram-negative E. coli, gram-positive facultative anaerobe cocci Staphylococcus lugdensis, Micrococcus halobius, and Stomatococcus mucilaginosus as subjects of study were chosen. LEDs with spectrum maxima at 405 nm (without any exogenous sensitizer) and 660 nm (in conjunction with methylene blue) and power densities of 23 mW/cm² and 5.7 mW/cm² accordingly as continuous light sources were chosen. Photosensitized light's affect by methylene blue was studied on E. coli only. The original scheme of experiment set up was developed. It permits one to increase expositions quantity in each experiment for more certain trend's construction over dose curves and decrease parasite flora sowing. As a result of accomplished studies it was established that blue low-coherent light have unalike weak light's dose depending suppressing effect on cocci whereas red low-coherent light have a moderate dose-depended suppressing effect at low irradiation doses and a moderate dose-depended stimulating effect at high irradiation doses on sensitized by MeBlue E. coli. For all ofthis, but Staphylococcus morphology changes were observed.

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Denis Ed. Popov, Elena S. Tuchina, Julia A. Chernova, Dmitry Podshibyakin, Dmitry V. Rudik, Maria Samsonova, Igor Gromov, and Valery V. Tuchin "The affect of low-coherent light on microbial colony forming ability and morphology of some gram-positive and gram-negative bacteria", Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); https://doi.org/10.1117/12.634773

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