Detection of biomolecules in complex media using surface plasmon resonance sensors

Michael R. Malone; Jean-Francois Masson; Margaret Barhnart; Stephen Beaudoin; Karl S. Booksh

doi:10.1117/12.630736

11 November 2005 Detection of biomolecules in complex media using surface plasmon resonance sensors

Michael R. Malone, Jean-Francois Masson, Margaret Barhnart, Stephen Beaudoin, Karl S. Booksh

Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 60070V (2005) https://doi.org/10.1117/12.630736
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

Detection of multiple biologically relevant molecules was accomplished at sub-ng/mL levels in highly fouling media using fiber- optic based surface plasmon resonance sensors. Myocardial infarction markers, myoglobin and cTnI, were quantified in full serum with limits of detection below 1 ng/mL. Biologically relevant levels are between 15-30 ng/mL and 1-5 ng/mL for myoglobin and cTnI respectively. Cytokines involved in chronic wound healing, Interleukin 1, Interleukin 6, and tumor necrosis factor α, were detected at around 1 ng/mL in cell culture media. Preliminary results in monitoring these cytokines in cell cultures expressing the cytokines were obtained. The protein diagnostic of spinal muscular atrophy, survival motor neuron protein, was quantified from cell lysate. To obtain such results in complex media, the sensor's stability to non-specific protein adsorption had to be optimized. A layer of the N-hydroxysuccinimide ester of 16-mercaptohexadecanoic acid is attached to the sensor. This layer optimizes the antibody attachment to the sensor while minimizing the non-specific signal from serum proteins.

Citation Download Citation

Michael R. Malone, Jean-Francois Masson, Margaret Barhnart, Stephen Beaudoin, and Karl S. Booksh "Detection of biomolecules in complex media using surface plasmon resonance sensors", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 60070V (11 November 2005); https://doi.org/10.1117/12.630736

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