Expresssion of bax/bcl-xl by low-power laser irradiation in the Amyloid Beta 25-35 induced apoptosis of PC12 cell

Lan Zhang; Da Xing

doi:10.1117/12.710708

27 October 2006 Expresssion of bax/bcl-xl by low-power laser irradiation in the Amyloid Beta 25-35 induced apoptosis of PC12 cell

Lan Zhang, Da Xing

Author Affiliations +

Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60473E (2006) https://doi.org/10.1117/12.710708
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Apoptosis has been reported as a contributing pathophysiological mechanism of Alzheimer's disease (AD). Recently, the anti-apoptosis function of low-power laser irradiation (LPLI) was proposed, suggesting LPLI may become a new means for AD therapy. In this study, we aimed to demonstrate the anti-apoptosis function of LPLI at molecular level. Aβ_25-35 was used to induce apoptosis of PC12 cell, and then the cells were dealt with LPLI. After irradiation, the molecular level of apoptosis was detected by quantifying the bax I bcl-xl mRNA ratio using a highly sensitive and quantitative polymerase chain reaction (QT-PCR) technique. The primary results show that the bax Ibcl-xl mRNA ratio of the PC12 cell treated with Aβ_25-35 was decreased by LPLI, demonstrating the anti-apoptosis function of LPLI at molecular level.

Citation Download Citation

Lan Zhang and Da Xing "Expresssion of bax/bcl-xl by low-power laser irradiation in the Amyloid Beta 25-35 induced apoptosis of PC12 cell", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60473E (27 October 2006); https://doi.org/10.1117/12.710708

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