Dynamics of cells function on laser cell-chip system

Toshihiro Kushibiki; Tomoko Sano; Katsunori Ishii; Sachiko Yoshihashi-Suzuki; Kunio Awazu

doi:10.1117/12.646188

25 February 2006 Dynamics of cells function on laser cell-chip system

Toshihiro Kushibiki, Tomoko Sano, Katsunori Ishii, Sachiko Yoshihashi-Suzuki, Kunio Awazu

Proceedings Volume 6080, Advanced Biomedical and Clinical Diagnostic Systems IV; 60800B (2006) https://doi.org/10.1117/12.646188
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A new type of cell-cultivation system based on laser processing has been developed for the on-chip cultivation of living cells. We introduce a "laser cell-chip", on which migration of cells, such as stem cells, tumor cells or immunocompetent cells, can be observed. A sheet prepared from epoxy resin was processed by KrF excimer laser (248 nm, 1.6 J/cm²) for preparation of microgrooved surfaces with various groove width, spacing, and depth. A laser cell-chip can make kinetic studies of cell migration depending on the concentration gradient of a chemoattractant. In this study, megakaryocytes were used for the migration on a groove of laser cell-chip by the concentration gradient of the stromal cell derived factor 1 (SDF-1/CXCL12). SDF-1/CXCL12 plays an important and unique role in the regulation of stem/progenitor cell trafficking. A megakaryocyte was migrated on a groove of laser cell-chip depending on the optical concentration gradient of SDF-1/CXCL12. Since SDF-1/CXCL12-induced migration of mature megakaryocyte was known to increase the platelet production in the bone marrow extravascular space, the diagnosis of cell migration on laser cell-chip could provide a new strategy to potentially reconstitute hematopoiesis and avoid life-threatening hemorrhage after myelosuppression or bone marrow failure.

Citation Download Citation

Toshihiro Kushibiki, Tomoko Sano, Katsunori Ishii, Sachiko Yoshihashi-Suzuki, and Kunio Awazu "Dynamics of cells function on laser cell-chip system", Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 60800B (25 February 2006); https://doi.org/10.1117/12.646188

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