Multiphoton imaging of corneal tissue with near-infrared femtosecond laser pulses: corneal optical tomography and its use in refractive surgery

Bao-Gui Wang; Karsten Koenig; Iris Riemann; Harald Schubert; Karl-Juergen Halbhuber

doi:10.1117/12.640594

23 February 2006 Multiphoton imaging of corneal tissue with near-infrared femtosecond laser pulses: corneal optical tomography and its use in refractive surgery

Bao-Gui Wang, Karsten Koenig, Iris Riemann, Harald Schubert, Karl-Juergen Halbhuber

Author Affiliations +

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 60891M (2006) https://doi.org/10.1117/12.640594
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The two-photon-mediated autofluorescence and second harmonic generation (SHG) are acting as a novel diagnostic tool to perform tissue optical tomography with submicron resolution. The three-dimensional corneal ultrastructure of whole depth can be probed without any staining or mechanical slicing. Compared with photodisruptive surgical effects occurring at TW/cm² light intensity, multiphoton imaging can be induced at MW-GW/cm² photon intensity. The multiphoton microscopy based on nonlinear absorption of femtosecond laser pulses at the wavelength of 715-930nm emitted from solid-state Ti: sapphire system is being used as a precise non-invasive monitoring tool to determine the interest of region, to visualize and to verify the outcomes in the invivo intrastromal laser nanosurgery. More interesting, the activated keratocytes have been also observed in-vivo 24 hours after the laser nanosurgery with this system. Overall, these data suggest that multiphoton microscopy is a highly sensitive and promising technique for studying the morphometric properties of the microstructure of the corneal tissue and for assessing the intrastromal nanosurgery. With the help of the multiphoton-mediated imaging, the next generation of laser refractive surgery approaches based on the nonamplified femtosecond lasers with higher precision and less complications are being evaluated systematically.

Citation Download Citation

Bao-Gui Wang, Karsten Koenig, Iris Riemann, Harald Schubert, and Karl-Juergen Halbhuber "Multiphoton imaging of corneal tissue with near-infrared femtosecond laser pulses: corneal optical tomography and its use in refractive surgery", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60891M (23 February 2006); https://doi.org/10.1117/12.640594

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