Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements

Zhengxi Huang; Dongmei Ji; Sufan Wang; Andong Xia; Felix Koberling; Matthias Patting; Rainer Erdmann

doi:10.1117/12.650492

27 February 2006 Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements

Zhengxi Huang, Dongmei Ji, Sufan Wang, Andong Xia, Felix Koberling, Matthias Patting, Rainer Erdmann

Author Affiliations +

Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920Y (2006) https://doi.org/10.1117/12.650492
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The triplet state characteristics of Cy5 molecule structurally related to the trans-cis isomerization are studied by means of ensemble and single molecule measurements. Several new deactivations of the singlet state are identified. The delayed fluorescence and phosphorescence from Cy5 molecule were observed. Spectral evidences show that the dim state could originate from the cis-Cy5 with lower excitation rate. The power dependence analysis for the same individual Cy5 molecule indicates an efficient reverse intersystem crossing happened while the T₁-T_n absorption is in resonance with the excitation wavelength.

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Zhengxi Huang, Dongmei Ji, Sufan Wang, Andong Xia, Felix Koberling, Matthias Patting, and Rainer Erdmann "Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920Y (27 February 2006); https://doi.org/10.1117/12.650492

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