Labeling of subcellular redox potential with dopamine-conjugated quantum dots

Stephen E. Bradforth; Samuel J. Clarke; C. Annette Hollmann; Daniel Bahcheli; Jay L. Nadeau

doi:10.1117/12.663332

27 March 2006 Labeling of subcellular redox potential with dopamine-conjugated quantum dots

Stephen E. Bradforth, Samuel J. Clarke, C. Annette Hollmann, Daniel Bahcheli, Jay L. Nadeau

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60960O (2006) https://doi.org/10.1117/12.663332
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Semiconductor quantum dots (QDs) possess highly reactive electrons and holes after photoexcitation. The energy of these electrons and holes can be deliberately modulated by attaching the QD to an electron donor or acceptor. This eliminates (quenches) QD fluorescence, as well as affecting the ability of the QD to oxidize or reduce common biomolecules such as glutathione and DNA. This greatly alters the fluorescent properties and toxicity of such QDs inside cells. In this work, we show that a specific electron donor, the neurotransmitter dopamine, yields redox-sensitive conjugates when attached to at least some colors of CdSe/ZnS QDs. The potential for the use of such conjugates as sensors, and the implications for enhanced toxicity in such conjugates are discussed.

Citation Download Citation

Stephen E. Bradforth, Samuel J. Clarke, C. Annette Hollmann, Daniel Bahcheli, and Jay L. Nadeau "Labeling of subcellular redox potential with dopamine-conjugated quantum dots", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960O (27 March 2006); https://doi.org/10.1117/12.663332

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