A microfabricated bio-sensor for erythrocytes deformability and volume distributions analysis

Avishay Bransky; Natanel Korin; Yael Nemirovski; Uri Dinnar

doi:10.1117/12.695562

14 December 2006 A microfabricated bio-sensor for erythrocytes deformability and volume distributions analysis

Avishay Bransky, Natanel Korin, Yael Nemirovski, Uri Dinnar

Proceedings Volume 6416, Biomedical Applications of Micro- and Nanoengineering III; 64160O (2006) https://doi.org/10.1117/12.695562
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2006, Adelaide, Australia

Abstract

The deformability of erythrocytes is of great importance for oxygen delivery in the microcirculation. Reduced RBC deformability is associated with several types of hemolytic anaemias, malaria, sepsis and diabetes. Aging of erythrocytes is also associated with loss of deformability as well as reduction in cell volume. An automated rheoscope has been developed, utilizing a microfabricated glass flow cell, high speed camera and advanced image-processing software. RBCs suspended in a high viscosity medium were filmed flowing through a microchannel. The system produces valuable data such as velocity profiles of RBCs, spatial distribution within the microchannel, cell volume and deformation index (DI) curves. The variation of DI across the channel height, due to change in shear stress, was measured for the first time. Such DI curves were obtained for normal and Thalassemia RBCs and their diagnostic potential was demonstrated. The spatial distribution and velocity of RBCs and rigid microspheres were measured. Both RBC and rigid spheres showed enhanced inward lateral migration, however the RBCs form a depletion region at the center of flow. The volume and surface area of the flowing cells have been estimated based on a fluid mechanics model and experimental results and fell within the normal range. Hence, the system developed, provides means for examining the behavior of individual RBCs in microchannels, and may serve as a microfabricated diagnostic device for deformability and volume measurements.

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Avishay Bransky, Natanel Korin, Yael Nemirovski, and Uri Dinnar "A microfabricated bio-sensor for erythrocytes deformability and volume distributions analysis", Proc. SPIE 6416, Biomedical Applications of Micro- and Nanoengineering III, 64160O (14 December 2006); https://doi.org/10.1117/12.695562

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KEYWORDS

Microfabrication

Image processing

Glasses

Image segmentation

Particles

Blood

Biological research

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