Imaging living cells with a combined high-resolution multi-photon-acoustic microscope

Selma Schenkl; Eike Weiss; Martin Stark; Frank Stracke; Iris Riemann; Robert Lemor; Karsten König

doi:10.1117/12.702391

13 February 2007 Imaging living cells with a combined high-resolution multi-photon-acoustic microscope

Selma Schenkl, Eike Weiss, Martin Stark, Frank Stracke, Iris Riemann, Robert Lemor, Karsten König

Proceedings Volume 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; 64372A (2007) https://doi.org/10.1117/12.702391
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

With increasing demand for in-vivo observation of living cells, microscope techniques that do not need staining become more and more important. In this talk we present a combined multi-photon-acoustic microscope with the possibility to measure synchronously properties addressed by ultrasound and two-photon fluorescence. Ultrasound probes the local mechanical properties of a cell, while the high resolution image of the two-photon fluorescence delivers insight in cell morphology and activity. In the acoustic part of the microscope an ultrasound wave, with a frequency of GHz, is focused by an acoustic sapphire lens and detected by a piezo electric transducer assembled to the lens. The achieved lateral resolution is in the range of 1&mgr;m. Contrast in the images arises mainly from the local absorption of sound in the cells, related to properties, such as mass density, stiffness and viscose damping. Additionally acoustic microscopy can access the cell shape and the state of the cell membrane as it is a intrinsic volume scanning technique.The optical part bases on the emission of fluorescent biomolecules naturally present in cells (e.g. NAD(P)H, protophorphyrin IX, lipofuscin, melanin). The nonlinear effect of two-photon absorption provides a high lateral and axial resolution without the need of confocal detection. In addition, in the near-IR cell damages are drastically reduced in comparison to direct excitation in the visible or UV. Both methods can be considered as minimal invasive, as they relay on intrinsic contrast mechanisms and dispense with the need of staining. First results on living cells are presented and discussed.

Citation Download Citation

Selma Schenkl, Eike Weiss, Martin Stark, Frank Stracke, Iris Riemann, Robert Lemor, and Karsten König "Imaging living cells with a combined high-resolution multi-photon-acoustic microscope", Proc. SPIE 6437, Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, 64372A (13 February 2007); https://doi.org/10.1117/12.702391

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Acoustics

Microscopes

Ultrasonography

Luminescence

Microscopy

Sapphire

Imaging systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years