Supported lipid bilayer membranes on SiO2 and TiO2: substrate effects on membrane formation and shape transformation

Ryugo Tero; Toru Ujihara; Tsuneo Urisu

doi:10.1117/12.733628

25 September 2007 Supported lipid bilayer membranes on SiO₂ and TiO₂: substrate effects on membrane formation and shape transformation

Ryugo Tero, Toru Ujihara, Tsuneo Urisu

Author Affiliations +

Proceedings Volume 6769, Nanosensing: Materials, Devices, and Systems III; 67690J (2007) https://doi.org/10.1117/12.733628
Event: Optics East, 2007, Boston, MA, United States

Abstract

Lipid bilayer membranes deposited on solid surfaces are called "supported planar bilayers" (SPBs), and expected to be an effective cell-membrane-mimicking model system in vitro. We have investigated the influence of the substrate surface properties on the SPB formation process and on the photo-induced shape transformation of bilayers, by means of atomic force microscopy and fluorescence microscopy. The SPB of dipalmitoleoylphosphatidylcholine was formed on SiO₂/Si(100) surfaces and rutile-TiO₂(100) surface by the vesicle fusion method. On the SiO₂ surface, one or a few adsorbed vesicles can transform to a SPB resulting in a small bilayer patches. The SPB formation rate was accelerated on thermally treated SiO₂ surfaces, which had less hydrophilicity, but the initial SPB formation process did not change. On the TiO₂(100), the surface was completely covered with the adsorbed vesicles prior to the SPB formation, and the planar bilayer was obtained only if the lipid concentration in the suspension was sufficiently high. Photo-induced activation of molecular motion through the fluorescence dye excitation achieved the area-selective SPB formation from the adsorbed vesicular layers with small SPB domains on the TiO₂(100). This photo-activation transformed the SPB shapes threedimensionally on the SiO₂ and TiO₂ surfaces in different way.

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Ryugo Tero, Toru Ujihara, and Tsuneo Urisu "Supported lipid bilayer membranes on SiO₂ and TiO₂: substrate effects on membrane formation and shape transformation", Proc. SPIE 6769, Nanosensing: Materials, Devices, and Systems III, 67690J (25 September 2007); https://doi.org/10.1117/12.733628

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