New approach on evaluation of liposome release property with electric impedance measuring method

Guoming Chen; Zhongwei Jiang; Makato Yoshimoto; Yafang Luo; Yunlong Wei

doi:10.1117/12.783960

9 January 2008 New approach on evaluation of liposome release property with electric impedance measuring method

Guoming Chen, Zhongwei Jiang, Makato Yoshimoto, Yafang Luo, Yunlong Wei

Proceedings Volume 6794, ICMIT 2007: Mechatronics, MEMS, and Smart Materials; 67943J (2008) https://doi.org/10.1117/12.783960
Event: ICMIT 2007: Mechatronics, MEMS, and Smart Materials, 2007, Gifu, Japan

Abstract

The purpose of this study is to develop a novel method for evaluation of the liposomes' release property by detecting the electric impedance change in the liposome solution. Liposomes encapsulating calcein (calcein-liposomes) and de-ionized water (white-liposomes) were prepared and ultrasound was employed as the stimulus in order to release the calcein from liposomes. The impedance change of calcein-liposomes in de-ionized water was measured before and after ultrasound treatment, as well as that of white-liposomes in de-ionized water and in calcein solutions for comparison. Besides, the release of calcein-liposome suspensions was evaluated simultaneously by spectrofluorometer. Results showed that the impedance of white-liposomes would change greatly when it was exposed to ultrasound and it was related to the total ion concentration of the sample. The release rate of calcein calculated with electric impedances showed a good agreement to that calculated with fluorescence intensities. It indicates that the electric impedance measuring method has high potential to estimate the release rate as the fluorescence analysis do, and might be used for non-fluorescent encapsulated liposomes which could not be evaluated by fluorescence method.

Citation Download Citation

Guoming Chen, Zhongwei Jiang, Makato Yoshimoto, Yafang Luo, and Yunlong Wei "New approach on evaluation of liposome release property with electric impedance measuring method", Proc. SPIE 6794, ICMIT 2007: Mechatronics, MEMS, and Smart Materials, 67943J (9 January 2008); https://doi.org/10.1117/12.783960

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