An immuno-biosensor system based on quartz crystal microbalance for avian influenza virus detection

Shengping Liu; Guoming Chen; Qi Zhou; Yunlong Wei

doi:10.1117/12.783971

9 January 2008 An immuno-biosensor system based on quartz crystal microbalance for avian influenza virus detection

Shengping Liu, Guoming Chen, Qi Zhou, Yunlong Wei

Proceedings Volume 6794, ICMIT 2007: Mechatronics, MEMS, and Smart Materials; 67943P (2008) https://doi.org/10.1117/12.783971
Event: ICMIT 2007: Mechatronics, MEMS, and Smart Materials, 2007, Gifu, Japan

Abstract

For the quick detection of Avian Influenza Virus (AIV), a biosensor based on Quartz Crystal Microbalance (QCM) was fabricated according to the specific bonding principle between antibody and antigen. Staphylococcal Protein A (SPA) was extracted from Staphylococcus and purified. Then SPA was coated on the surface of QCM for immobilizing AIV monoclonal antibodies. The use of AIV monoclonal antibody could enhance the specificity of the immuno-biosensor. A multi-channel piezoelectricity detection system for the immuno-biosensor was developed. The system can work for the quick detection of AIV antigen in the case of the entirely aqueous status owe to one special oscillating circuit designed in this work. The optimum conditions of SPA coating and AIV monoclonal antibody immobilization were investigated utilizing the multi-channel detection system. The preliminary application of the immuno-biosensor system for detection of AIV was evaluated. Results indicate that the immuno-biosensor system can detect the AIV antigens with a linear range of 3-200ng/ml. The system can accomplish the detection of AIV antigens around 40 minutes.

Citation Download Citation

Shengping Liu, Guoming Chen, Qi Zhou, and Yunlong Wei "An immuno-biosensor system based on quartz crystal microbalance for avian influenza virus detection", Proc. SPIE 6794, ICMIT 2007: Mechatronics, MEMS, and Smart Materials, 67943P (9 January 2008); https://doi.org/10.1117/12.783971

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available