A novel biosensor for rapid identification of high temperature resistant species

Lingrui Zhang; Da Xing; Xiaoming Zhou

doi:10.1117/12.760225

26 November 2007 A novel biosensor for rapid identification of high temperature resistant species

Lingrui Zhang, Da Xing, Xiaoming Zhou

Proceedings Volume 6830, Advanced Sensor Systems and Applications III; 683027 (2007) https://doi.org/10.1117/12.760225
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

In this paper, a novel biosensor technique for identification of high temperature resistant species based on quantitative measurement of delayed fluorescence (DF) is described. The biosensor, which uses light-emitting diode lattice as excitation light source, is portable and can detect DF emission from plants in vivo. Compared with its primary version in our previous report, the biosensor presented here can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light response curves of DF intensity in a programmed mode. The testing of the improved biosensor has been made in two maize species (Zea May L.) after high temperature treatment. Contrast evaluations of the effects of heat stress on seedlings photosynthesis were made from measurements of net photosynthesis rate (Pn) based on consumption of CO₂. Current testing has demonstrated that the DF intensity well correlates with Pn in each plant species after heat stress. We thus conclude that the DF technique is a breakthrough to traditional strategy of identifying the differences in heat tolerance based on gas exchange, and can provide a reliable approach for rapid and non-invasive determination of the effects of heat stress on photosynthesis and identification of high temperature resistant species.

Citation Download Citation

Lingrui Zhang, Da Xing, and Xiaoming Zhou "A novel biosensor for rapid identification of high temperature resistant species", Proc. SPIE 6830, Advanced Sensor Systems and Applications III, 683027 (26 November 2007); https://doi.org/10.1117/12.760225

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