Accurate three-dimensional detection of micro-particles by means of digital holographic microscopy

Maciej Antkowiak; Natacha Callens; Cédric Schockaert; Catherine Yourassowsky; Frank Dubois

doi:10.1117/12.781210

25 April 2008 Accurate three-dimensional detection of micro-particles by means of digital holographic microscopy

Maciej Antkowiak, Natacha Callens, Cédric Schockaert, Catherine Yourassowsky, Frank Dubois

Proceedings Volume 6995, Optical Micro- and Nanometrology in Microsystems Technology II; 699514 (2008) https://doi.org/10.1117/12.781210
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

We present a numerical technique for refocusing and three-dimensional localization of micron-size particles observed in a digital holographic microscope working in transmission. We use Fourier method for the extraction of complex amplitude from the single exposition digital holograms. The three dimensional localization of objects is performed using the focus plane determination method based on the integrated amplitude modulus. We apply the refocusing criterion locally for each pixel, using small overlapping windows, in order to obtain a synthetic image in which all objects are refocused independent from their refocusing distance. We perform image segmentation and object detection using both the synthetic refocused image and the value of refocusing criterion, which allows us to obtain a high detection efficiency with very low number of false detections. While the lateral precision of localization is determined by the optical resolution of the setup, the vertical accuracy depends on the parameters of the digital holographic reconstruction. We improve the accuracy of vertical localization using an additional refining procedure in which each particle is treated separately. We analyze the robustness and accuracy of our approach and present its successful implementation in particle flow experiments.

Citation Download Citation

Maciej Antkowiak, Natacha Callens, Cédric Schockaert, Catherine Yourassowsky, and Frank Dubois "Accurate three-dimensional detection of micro-particles by means of digital holographic microscopy", Proc. SPIE 6995, Optical Micro- and Nanometrology in Microsystems Technology II, 699514 (25 April 2008); https://doi.org/10.1117/12.781210

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