Accelerated adhesion of grafted skins by laser-induced stress wave-based gene transfer of hepatocyte growth factor

Kazuya Aizawa; Shunichi Sato; Daizoh Saitoh; Hitoshi Tsuda; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.808663

19 February 2009 Accelerated adhesion of grafted skins by laser-induced stress wave-based gene transfer of hepatocyte growth factor

Kazuya Aizawa, Shunichi Sato, Daizoh Saitoh, Hitoshi Tsuda, Hiroshi Ashida, Minoru Obara

Author Affiliations +

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 71610L (2009) https://doi.org/10.1117/12.808663
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

In our previous study, we delivered plasmid DNA coding for human hepatocyto growth factor (hHGF) to rat skin grafts based on laser-induced stress wave (LISW), by which production of CD31-positive cells in the grafted skins was found to be enhanced, suggesting improved angiogenesis. In this study, we validated the efficacy of this method to accelerate adhesion of grafted skins; reperfusion and reepithelialization in the grafted skins were examined. As a graft, dorsal skin of a rat was exsected and its subcutaneous fat was removed. Plasmid DNA expression vector for hHGF was injected into the graft; on its back surface a laser target with a transparent sheet for plasma confinement was placed, and irradiated with three nanosecond laser pulses at a laser fluence of 1.2 J/cm2 (532 nm; spot diameter, 3 mm) to generate LISWs. After the application of LISWs, the graft was transplanted onto its donor site. We evaluated blood flow by laser Doppler imaging and analyzed reepithelialization based on immunohistochemistry as a function of postgrafting time. It was found that both reperfusion and reepithelialization were significantly enhanced for the grafts with gene transfection than for normal grafts; reepithelialization was completed within 7 days after transplantation with the transfected grafts. These findings demonstrate that adhesion of grafted skins can be accelerated by delivering HGF gene to the grafts based on LISWs.

Citation Download Citation

Kazuya Aizawa, Shunichi Sato, Daizoh Saitoh, Hitoshi Tsuda, Hiroshi Ashida, and Minoru Obara "Accelerated adhesion of grafted skins by laser-induced stress wave-based gene transfer of hepatocyte growth factor", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71610L (19 February 2009); https://doi.org/10.1117/12.808663

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KEYWORDS

Skin

Transplantation

Tissues

Blood circulation

Doppler effect

Wound healing

Blood

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