Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells

Ying-ying Lu; Tong-sheng Chen; Jun-Le Qu

doi:10.1117/12.807255

24 February 2009 Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells

Ying-ying Lu, Tong-sheng Chen, Jun-Le Qu

Author Affiliations +

Proceedings Volume 7178, Biophotonics and Immune Responses IV; 71780J (2009) https://doi.org/10.1117/12.807255
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways. However, the molecular mechanisms are not well understood. This study was investigated in human lung adenocarconoma ASTC-a-1 cell line and aimed to determine whether the apoptotic process was mediated by Bax activation and translocation during DHA-induced apoptosis. In this study, DHA induced a time-dependent apoptotic cell death, which was assayed by Cell Counting Kit (CCK-8) and Hoechst 33258 staining. Detection of Bax aggregation and translocation to mitochondria was observed in living cells which were co-transfected with GFP-Bax and Dsred-mito plasmid using confocal fluorescence microscope technique. Overall, these results demonstrated that Bax activation and translocation to mitochondria occurred during DHA-induced apoptosis.

Citation Download Citation

Ying-ying Lu, Tong-sheng Chen, and Jun-Le Qu "Bax translocation into mitochondria during dihydroartemisinin(DHA)-induced apoptosis in human lung adenocarcinoma cells", Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780J (24 February 2009); https://doi.org/10.1117/12.807255

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