Real-time optical imaging of the interaction of epidermal growth factor and its receptor in living cells

Qiaoya Lin; Liang Wang; Shaoqun Zeng; Zhihong Zhang; Gang Zheng

doi:10.1117/12.811372

24 February 2009 Real-time optical imaging of the interaction of epidermal growth factor and its receptor in living cells

Qiaoya Lin, Liang Wang, Shaoqun Zeng, Zhihong Zhang, Gang Zheng

Author Affiliations +

Proceedings Volume 7178, Biophotonics and Immune Responses IV; 71780P (2009) https://doi.org/10.1117/12.811372
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Fluorescence resonance energy transfer (FRET) has been widely used in biology in recent years, and permits high spatial resolution assays of protein-protein interactions in living cells. Here, we first use the FRET technique to real-time observe the binding of EGF to EGFR on the surface of A549 cells and EGFR-GFP-ldlA7 cells, and continuously monitor this reaction for 1 hour. In addition, this is the first direct evidence that FRET occurred between different proteins which are in the intramembrane and extramembrane, respectively.

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Qiaoya Lin, Liang Wang, Shaoqun Zeng, Zhihong Zhang, and Gang Zheng "Real-time optical imaging of the interaction of epidermal growth factor and its receptor in living cells", Proc. SPIE 7178, Biophotonics and Immune Responses IV, 71780P (24 February 2009); https://doi.org/10.1117/12.811372

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KEYWORDS

Fluorescence resonance energy transfer

Receptors

Green fluorescent protein

Signal detection

Visualization

Bandpass filters

Molecules

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