Research on microfluidic chip and imaging system used to measure Ca2+ in cell

Wei Zhou; Sixiang Zhang; Dugang Ran; Bao Liu

doi:10.1117/12.835488

6 August 2009 Research on microfluidic chip and imaging system used to measure Ca²⁺ in cell

Wei Zhou, Sixiang Zhang, Dugang Ran, Bao Liu

Proceedings Volume 7384, International Symposium on Photoelectronic Detection and Imaging 2009: Advances in Imaging Detectors and Applications; 73841A (2009) https://doi.org/10.1117/12.835488
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

The microfluidic fluorescence detecting system which is used to measure the concentration of Ca²+ had been designed. On the microfluidic chip we designed, cell-dyeing, cell fostering, reagent injecting and other operations can be completed. The monochromatic light came from optical monochromator which can emit continuous spectrum was used to excitated the fluorescent probe in the cell, then the fluorescence signal and image were sampled by the PMT and CCD, at last the data was processed and the content of Ca2+ in the cell was figured out by using the fluorescence ratio method. Meanwhile, by using the system, the dynamic curve of [Ca2+]1 in cell was given after being stimulated by high K⁺. The precise result verifies that the system is stable and credible and it meets the requirement of detecting [Ca²+]i in live cells in the filed of physiology.

Citation Download Citation

Wei Zhou, Sixiang Zhang, Dugang Ran, and Bao Liu "Research on microfluidic chip and imaging system used to measure Ca²⁺ in cell", Proc. SPIE 7384, International Symposium on Photoelectronic Detection and Imaging 2009: Advances in Imaging Detectors and Applications, 73841A (6 August 2009); https://doi.org/10.1117/12.835488

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