Optical chromatographic sample fractionation

Alex Terray; Joseph D. Taylor; Sean J. Hart

doi:10.1117/12.828147

20 August 2009 Optical chromatographic sample fractionation

Alex Terray, Joseph D. Taylor, Sean J. Hart

Author Affiliations +

Proceedings Volume 7400, Optical Trapping and Optical Micromanipulation VI; 74000O (2009) https://doi.org/10.1117/12.828147
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

Optical Chromatography involves the elegant combination of opposing optical and fluid drag forces on colloidal samples within microfluidic environments to both measure analytical differences and fractionate injected samples. Particles that encounter the focused laser beam are trapped axially along the beam and are pushed upstream from the laser focal point to rest at a point where the optical and fluid forces on the particle balance. In our recent devices particles are pushed into a region of lower microfluidic flow, where they can be retained and fractionated. Because optical and fluid forces are sensitive to differences in the physical and chemical properties of a sample, differences between samples and thus separations are possible. An optical chromatography beam focused to completely fill a fluid channel is operated as an optically tunable filter for the separation of polymeric/colloidal and biological samples. We demonstrate this technique coupled with an advanced microfluidic platform and show how it can be used as an effective method to fractionate particles in an injected multi-component sample. Our advanced microfluidic design accommodates three lasers simultaneously to effectively create a sequential cascade optical chromatographic separation system.

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Alex Terray, Joseph D. Taylor, and Sean J. Hart "Optical chromatographic sample fractionation", Proc. SPIE 7400, Optical Trapping and Optical Micromanipulation VI, 74000O (20 August 2009); https://doi.org/10.1117/12.828147

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