Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy

Patrick Nuernberger; Kevin F. Lee; Adeline Bonvalet; Antigoni Alexandrou; Marten H. Vos; Manuel Joffre

doi:10.1117/12.843672

25 February 2010 Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy

Patrick Nuernberger, Kevin F. Lee, Adeline Bonvalet, Antigoni Alexandrou, Marten H. Vos, Manuel Joffre

Author Affiliations +

Proceedings Volume 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry; 75600F (2010) https://doi.org/10.1117/12.843672
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We report on ultrafast visible pump/mid-infrared probe spectroscopy of the carboxy form of heme proteins by employing the recently developed chirped-pulse upconversion technique, which allows both high resolution and sensitivity over an extremely broad spectral range. Commonly, the bleach signal due to ligand dissociation and the incipient docking-site absorption signal, being about 200 cm^-1 apart and differing by more than an order of magnitude in absorptivity, are studied in separate experiments. We here monitor them simultaneously, allowing a direct observation and a concurrent analysis of the initial processes after photoinduced ligand dissociation, for instance, the formation of hot vibrational bands.

Citation Download Citation

Patrick Nuernberger, Kevin F. Lee, Adeline Bonvalet, Antigoni Alexandrou, Marten H. Vos, and Manuel Joffre "Simultaneous observation of ultrafast ligand dissociation and docking-site trapping in heme proteins using upconversion infrared spectroscopy", Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600F (25 February 2010); https://doi.org/10.1117/12.843672

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