Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy

Toyohiko Yamauchi; Norikazu Sugiyama; Takashi Sakurai; Hidenao Iwai; Yutaka Yamashita

doi:10.1117/12.841008

24 February 2010 Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy

Toyohiko Yamauchi, Norikazu Sugiyama, Takashi Sakurai, Hidenao Iwai, Yutaka Yamashita

Author Affiliations +

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700X (2010) https://doi.org/10.1117/12.841008
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very lowcoherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.

Citation Download Citation

Toyohiko Yamauchi, Norikazu Sugiyama, Takashi Sakurai, Hidenao Iwai, and Yutaka Yamashita "Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700X (24 February 2010); https://doi.org/10.1117/12.841008

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