Non-negative matrix factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing

Anne-Sophie Montcuquet; Lionel Hervé; Jean-Marc Dinten; Jérôme I. Mars

doi:10.1117/12.840648

26 February 2010 Non-negative matrix factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing

Anne-Sophie Montcuquet, Lionel Hervé, Jean-Marc Dinten, Jérôme I. Mars

Author Affiliations +

Proceedings Volume 7573, Biomedical Applications of Light Scattering IV; 757314 (2010) https://doi.org/10.1117/12.840648
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Fluorescence optical imaging use one or several (in multiplexing) injected fluorescent markers which specifically bind to targeted compounds. Near infrared light illuminates the region of interest and the emitted fluorescence is analyzed to localize fluorescence sources. A spectroscopic approach and a separation source method (Nonnegative matrix factorization) are explored to separate different fluorescence sources and remove the unwanted biological tissues autofluorescence. We present unmixing results on overlapping spectra of interest, and show that autofluorescence removal improves Fluorescent Diffuse Optical Tomography.

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Anne-Sophie Montcuquet, Lionel Hervé, Jean-Marc Dinten, and Jérôme I. Mars "Non-negative matrix factorization: a blind sources separation method applied to optical fluorescence spectroscopy and multiplexing", Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 757314 (26 February 2010); https://doi.org/10.1117/12.840648

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