This paper studies a new method for chromosome pairing of amphioxus using digital image processing technology,
selects chromosome banding image of amphioxus Branchiostoma belcheri tsingtauense as experimental material,
compares and analyzes objective data such as perimeter, long axis and short axis of the chromosome, and realizes
chromosome pairing of amphioxus with Reference to band feature of Laplace and Sobel edge detection as pairing
supplement. According to objective data obtained from the program, this method improves the subjective pairing method
which uses image processing software to measure long arm and short arm of the chromosome. The results show that the
pairing method is effective, accurate and practical. This study has important theoretical and practical significance for
further study of chromosome gene mapping and cell genetics of amphioxus.
|