DNA aptamer functionalized zinc oxide field effect transistors for liquid state selective sensing of small molecules

Joshua A. Hagen; Sang Nyon Kim; Burhan Bayraktaroglu; Nancy Kelley-Loughnane; Rajesh R. Naik; Morley O. Stone

doi:10.1117/12.860574

24 August 2010 DNA aptamer functionalized zinc oxide field effect transistors for liquid state selective sensing of small molecules

Joshua A. Hagen, Sang Nyon Kim, Burhan Bayraktaroglu, Nancy Kelley-Loughnane, Rajesh R. Naik, Morley O. Stone

Author Affiliations +

Proceedings Volume 7759, Biosensing III; 775912 (2010) https://doi.org/10.1117/12.860574
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

In this work, we show the use of single stranded DNA aptamers as selective biorecognition elements in a sensor based on a field effect transistor (FET) platform. Aptamers are chemically attached to the semiconducting material in the FET through the use of linker molecules and confirmed through atomic force microscopy and positive target detection. Highly selective sensing of a small molecule, riboflavin is shown down to the nano-molar level in zinc oxide FET and micro-molar level in a carbon nanotube FET. High selectivity is determined through the use of negative control target molecules with similar molecular structures as the positive control targets with little to no sensor response. The goal of this work is to develop a sensor platform where biorecognition elements can be used to functionalize an array of transistors for simultaneous sensing of multiple targets in biological fluids.

Citation Download Citation

Joshua A. Hagen, Sang Nyon Kim, Burhan Bayraktaroglu, Nancy Kelley-Loughnane, Rajesh R. Naik, and Morley O. Stone "DNA aptamer functionalized zinc oxide field effect transistors for liquid state selective sensing of small molecules", Proc. SPIE 7759, Biosensing III, 775912 (24 August 2010); https://doi.org/10.1117/12.860574

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