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The interaction between tri or tetravalent cerium ions and basic fibroblast growth factor (FGF-2) at 0.1-6: 1 molar ratio
under physiological condition was studied by fluorescence and CD spectrum. The different spectra alterations of FGF-2
induced by Ce3+ and Ce4+ showed that Ce3+ and Ce4+ caused different conformational changes of FGF-2 respectively,
though both of them destabilized the protein. The instability of FGF-2 in the presence of Ce3+ is involved in the oxidation
of its free cystein of protein, but that this treatment nearly does not affect the biological activity. As to Ce4+, it not only
induced the conformational changes of protein but also inhibits its activity in a dose-dependent manner, which could be
relative to the electrostatic repulsion between Ce4+ and its basic amino acid residues (pI=9.6) or the specific binding of
Ce4+ to deprotonated amino acid residues. The interesting results would be helpful to investigate the problem of the
stability of proteins.
Liwei Sun,Hao Feng,Rui Jiang,Liping Niu,Yu Song,Kai Feng, andChao Qi
"The effects of Ce3+ and Ce4+ on the stability of fibroblast growth factor-2", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784515 (8 November 2010); https://doi.org/10.1117/12.868223
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Liwei Sun, Hao Feng, Rui Jiang, Liping Niu, Yu Song, Kai Feng, Chao Qi, "The effects of Ce3+ and Ce4+ on the stability of fibroblast growth factor-2," Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784515 (8 November 2010); https://doi.org/10.1117/12.868223