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11 February 2011 Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids
Kevin Braeckmans, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek N. Sanders, Jo Demeester, Stefaan C. De Smedt
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Proceedings Volume 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 79080B (2011) https://doi.org/10.1117/12.889218
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
While extremely relevant to many life science fields, such as biomedical diagnostics and drug delivery, studies on the size of nanoparticulate matter dispersed in biofluids are missing due to a lack of suitable methods. Here we report that fluorescence single particle tracking (fSPT) with maximum entropy analysis is the first technique suited for accurate sizing of nanoparticles dispersed in biofluids, such as whole blood. After a thorough validation, the fSPT sizing method was applied to liposomes that have been under investigation for decades as nanocarriers for drugs. The tendency of these liposomes to form aggregates in whole blood was tested in vitro and in vivo. In addition, we have demonstrated that the fSPT sizing technique can be used for identifying and sizing natural cell-derived microparticles directly in plasma. fSPT sizing opens up the possibility to systematically study the size and aggregation of endogenous or exogenous nanoparticles in biofluids.
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Kevin Braeckmans, Kevin Buyens, Wim Bouquet-Geerardyn, Chris Vervaet, Philippe Joye, Filip De Vos, Laurent Plawinski, Loïc Doeuvre, Eduardo Angles-Cano, Niek N. Sanders, Jo Demeester, and Stefaan C. De Smedt "Fluorescence single particle tracking for sizing of nanoparticles in undiluted biological fluids", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080B (11 February 2011); https://doi.org/10.1117/12.889218
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KEYWORDS
Blood
Particles
Nanoparticles
Luminescence
Blood circulation
Diffusion
Biomedical optics
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