Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging

S. Montanha; S. Pratavieira; D. P. Jacomassi; A. N. S. Rastelli; V. S. Bagnato

doi:10.1117/12.909136

1 February 2012 Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging

S. Montanha, S. Pratavieira, D. P. Jacomassi, A. N. S. Rastelli, V. S. Bagnato

Proceedings Volume 8208, Lasers in Dentistry XVIII; 82080Q (2012) https://doi.org/10.1117/12.909136
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem® solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope® system. For image capture a scientific CCD color camera PixelFly® was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab®. Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching.

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S. Montanha, S. Pratavieira, D. P. Jacomassi, A. N. S. Rastelli, and V. S. Bagnato "Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging", Proc. SPIE 8208, Lasers in Dentistry XVIII, 82080Q (1 February 2012); https://doi.org/10.1117/12.909136

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KEYWORDS

Picosecond phenomena

Diffusion

Luminescence

Photoacoustic tomography

Photodynamic therapy

Teeth

CCD cameras

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