Three dimensional refractive index imaging with differential interference contrast microscopy

Htet Aung; Jared Buckley; Piotr Kostyk; Braulio Rodriguez; Shelley Phelan; M. Xu

doi:10.1117/12.910339

2 February 2012 Three dimensional refractive index imaging with differential interference contrast microscopy

Htet Aung, Jared Buckley, Piotr Kostyk, Braulio Rodriguez, Shelley Phelan, M. Xu

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82270G (2012) https://doi.org/10.1117/12.910339
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We report here a new approach based on an extension of the transport of the intensity equation for three dimensional refractive index imaging of a weak phase object from a series of images recorded by a differential interference contrast microscope at different focus (z-stack). Our method is first validated by imaging polystyrene spheres. We then apply this method to monitor in vivo apoptosis of human breast MCF7 epithelial cells. The potential applications are discussed at the end.

Citation Download Citation

Htet Aung, Jared Buckley, Piotr Kostyk, Braulio Rodriguez, Shelley Phelan, and M. Xu "Three dimensional refractive index imaging with differential interference contrast microscopy", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270G (2 February 2012); https://doi.org/10.1117/12.910339

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