The use of a handheld Raman system for virus detection

Chunyuan Song; Jeremy D. Driskell; Ralph A. Tripp; Yiping Cui; Yiping Zhao

doi:10.1117/12.918758

4 May 2012 The use of a handheld Raman system for virus detection

Chunyuan Song, Jeremy D. Driskell, Ralph A. Tripp, Yiping Cui, Yiping Zhao

Author Affiliations +

Proceedings Volume 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII; 83580I (2012) https://doi.org/10.1117/12.918758
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

The combination of surface enhanced Raman spectroscopy (SERS) with a handheld Raman system would lead to a powerful portable device for defense and security applications. The Thermo Scientific FirstDefender RM instrument is a 785-nm handheld Raman spectrometer intended for rapid field identification of unknown solid and liquid samples. Its sensitivity and effectiveness for SERS-based detection was initially confirmed by evaluating detection of 1,2-di(4- pyridyl)ethylene as a reporter molecule on a silver nanorod (AgNR) substrate, and the results are comparable to those from a confocal Bruker Raman system. As avian influenza A viruses (AIV) are recognized as an important emerging threat to public health, this portable handheld Raman spectrometer is used, for the first time, to detect and identify avian influenza A viruses using a multi-well AgNR SERS chip. The SERS spectra obtained had rich peaks which demonstrated that the instrument can be effectively used for SERS-based influenza virus detection. According to the SERS spectra, these different influenza viruses were distinguished from the negative control via the principal component analysis and by partial least squares-discriminate analysis. Together, these results show that the combination effective SERS substrates when combined with a portable Raman spectrometer provides a powerful field device for chemical and biological sensing.

Citation Download Citation

Chunyuan Song, Jeremy D. Driskell, Ralph A. Tripp, Yiping Cui, and Yiping Zhao "The use of a handheld Raman system for virus detection", Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 83580I (4 May 2012); https://doi.org/10.1117/12.918758

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