The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)

Sung Eun Kim; Su-Young Lee; Young-Pil Yun; Jae Yong Lee; Kyeongsoon Park; Deok-Won Lee; Hae-Ryong Song

doi:10.1117/12.979889

24 October 2012 The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)

Sung Eun Kim, Su-Young Lee, Young-Pil Yun, Jae Yong Lee, Kyeongsoon Park, Deok-Won Lee, Hae-Ryong Song

Author Affiliations +

Proceedings Volume 8548, Nanosystems in Engineering and Medicine; 85486B (2012) https://doi.org/10.1117/12.979889
Event: SPIE Nanosystems in Engineering + Medicine, 2012, Incheon, Korea, Republic of

Abstract

The purpose of this study was to develop alendronate (Aln)-eluting Ti substrates to induce osteogenic differentiation of human buccal fat cells (HBFCs). The surface of pristine Ti was modified by dopamine (DOPA) and then heparin was grafted onto the aminated Ti surfaces to achieve the Aln-eluting Ti system. Aln was subsequently immobilized on the surface of heparinized Ti (Hep-Ti). Pristine Ti and surface-modified-Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. Osteogenic differentiation of HBFCs on the surface of pristine-Ti, Hep-Ti, Aln (1 mg)/Hep-Ti, and Aln (5 mg)/Hep-Ti was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Successful immobilization of Aln on Hep-Ti was confirmed by XPS and contact angle. Aln/Hep-Ti showed the sustained release for up to 28 days. Additionally, HBFCs cultured on Aln/Hep-Ti substrates showed significantly induced ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. These results suggest that Aln-eluting Ti substrates have a potential effect on osteogenic differentiation of HBFCs and will be a promising material for bone regeneration.

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Sung Eun Kim, Su-Young Lee, Young-Pil Yun, Jae Yong Lee, Kyeongsoon Park, Deok-Won Lee, and Hae-Ryong Song "The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85486B (24 October 2012); https://doi.org/10.1117/12.979889

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KEYWORDS

Aluminum nitride

Aluminium phosphide

Calcium

Titanium

Bone

Magnesium

Stem cells

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