A fiber-laser-based stimulated Raman scattering spectral microscope

Keisuke Nose; Yasuyuki Ozeki; Tatsuya Kishi; Kazuhiko Sumimura; Yasuo Kanematsu; Kazuyoshi Itoh

doi:10.1117/12.2004275

22 February 2013 A fiber-laser-based stimulated Raman scattering spectral microscope

Keisuke Nose, Yasuyuki Ozeki, Tatsuya Kishi, Kazuhiko Sumimura, Yasuo Kanematsu, Kazuyoshi Itoh

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85880Y (2013) https://doi.org/10.1117/12.2004275
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) spectral microscopy is a powerful technique for label-free biological imaging because it allows us to distinguish chemical species with overlapping Raman bands. Here we present an SRS spectral microscope based only on fiber lasers (FL’s), which offer the possibilities of downsizing and simplification of the system. A femtosecond figure-8 Er-FL at a repetition rate of 54.4 MHz is used to generate pump pulses. After amplified by an Er doped fiber amplifier, Er-FL pulses are spectrally compressed to 2-ps second harmonic pulses. For generating Stokes pulses, a femtosecond Yb-FL pulses at a repetition rate of 27.2 MHz is used. Then these lasers are synchronized by a phase locked loop, which consists of a two-photon absorption photodetector, a loop filter, a phase modulator in the Er- FL cavity, and a piezo electric transducer in the Yb-FL cavity. The intensity noise of pump pulses is reduced by the collinear balanced detection (CBD) technique based on delay-and-add fiber lines. Experimentally, we confirmed that the intensity noise level of probe pulses was close to the shot noise limit. The Stokes pulses are introduced to a wavelength tunable band pass filter (BPF), which consists of a galvanomirror scanner, a 4-f optical system, a reflection grating, and a collimator. This system is able to scan the wavenumber from 2850 cm^-1 to 3100 cm^-1 by tuning the BPF. We succeeded in the spectral imaging of a mixture of polystyrene beads and poly(methyl methacrylate) beads.

Citation Download Citation

Keisuke Nose, Yasuyuki Ozeki, Tatsuya Kishi, Kazuhiko Sumimura, Yasuo Kanematsu, and Kazuyoshi Itoh "A fiber-laser-based stimulated Raman scattering spectral microscope", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880Y (22 February 2013); https://doi.org/10.1117/12.2004275

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available