The antibody-based magnetic microparticle immunoassay using p-FET sensing platform for Alzheimer's disease pathogenic factor

Chang-Beom Kim; Kwan-Soo Kim; Ki-Bong Song

doi:10.1117/12.2017797

20 May 2013 The antibody-based magnetic microparticle immunoassay using p-FET sensing platform for Alzheimer's disease pathogenic factor

Chang-Beom Kim, Kwan-Soo Kim, Ki-Bong Song

Author Affiliations +

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 887903 (2013) https://doi.org/10.1117/12.2017797
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

The importance of early Alzheimer’s disease (AD) detection has been recognized to diagnose people at high risk of AD. The existence of intra/extracellular beta-amyloid (Aβ) of brain neurons has been regarded as the most archetypal hallmark of AD. The existing computed-image-based neuroimaging tools have limitations on accurate quantification of nanoscale Aβ peptides due to optical diffraction during imaging processes. Therefore, we propose a new method that is capable of evaluating a small amount of Aβ peptides by using photo-sensitive field-effect transistor (p-FET) integrated with magnetic force-based microbead collecting platform and selenium(Se) layer (thickness ~700 nm) as an optical filter. This method demonstrates a facile approach for the analysis of Aβ quantification using magnetic force and magnetic silica microparticles (diameter 0.2~0.3 μm). The microbead collecting platform mainly consists of the p-FET sensing array and the magnet (diameter ~1 mm) which are placed beneath each sensing region of the p-FET, which enables the assembly of the Aβ antibody conjugated microbeads, captures the Aβ peptides from samples, measures the photocurrents generated by the Q-dot tagged with Aβ peptides, and consequently results in the effective Aβ quantification.

Citation Download Citation

Chang-Beom Kim, Kwan-Soo Kim, and Ki-Bong Song "The antibody-based magnetic microparticle immunoassay using p-FET sensing platform for Alzheimer's disease pathogenic factor", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 887903 (20 May 2013); https://doi.org/10.1117/12.2017797

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