Comparison of the efficiency control of mycotoxins by some optical immune biosensors

N. F. Slyshyk; N. F. Starodub

doi:10.1117/12.2044756

18 November 2013 Comparison of the efficiency control of mycotoxins by some optical immune biosensors

N. F. Slyshyk, N. F. Starodub

Proceedings Volume 9032, Biophotonics—Riga 2013; 903210 (2013) https://doi.org/10.1117/12.2044756
Event: 1st International Conference "Biophotonics Riga 2013", 2013, Riga, Latvia

Abstract

It was compared the efficiency of patulin control at the application of such optical biosensors which were based on the surface plasmon resonance (SPR) and nano-porous silicon (sNPS). In last case the intensity of the immune reaction was registered by measuring level of chemiluminescence (ChL) or photocurrent of nPS. The sensitivity of this mycotoxin determination by first type of immune biosensor was 0.05-10 mg/L Approximately the same sensitivity as well as the overall time analysis were demonstrated by the immune biosensor based on the nPS too. Nevertheless, the last type of biosensor was simpler in technical aspect and the cost of analysis was cheapest. That is why, it was recommend the nPS based immune biosensor for wide screening application and SPR one for some additional control or verification of preliminary obtained results. In this article a special attention was given to condition of sample preparation for analysis, in particular, micotoxin extraction from potao and some juices. Moreover, it was compared the efficiency of the above mentioned immune biosensors with such traditional approach of mycotoxin determination as the ELISA-method. In the result of investigation and discussion of obtained data it was concluded that both type of the immune biosensors are able to fulfill modern practice demand in respect sensitivity, rapidity, simplicity and cheapness of analysis.

Citation Download Citation

N. F. Slyshyk and N. F. Starodub "Comparison of the efficiency control of mycotoxins by some optical immune biosensors", Proc. SPIE 9032, Biophotonics—Riga 2013, 903210 (18 November 2013); https://doi.org/10.1117/12.2044756

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KEYWORDS

Biosensors

Biological research

Silicon

Chromatography

Gold

Proteins

Surface plasmons

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