Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action

Silvie Bernatova; Ota Samek; Zdenek Pilat; Mojmir Sery; Jan Jezek; Petr Jakl; Martin Siler; Vladislav Krzyzanek; Pavel Zemanek; Veronika Hola; Milada Dvorackova; Filip Ruzicka

doi:10.1117/12.2052538

8 May 2014 Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action

Silvie Bernatova, Ota Samek, Zdenek Pilat, Mojmir Sery, Jan Jezek, Petr Jakl, Martin Siler, Vladislav Krzyzanek, Pavel Zemanek, Veronika Hola, Milada Dvorackova, Filip Ruzicka

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 91291Y (2014) https://doi.org/10.1117/12.2052538
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Raman tweezers represents a unique method for identification of different microorganisms on the basis of Raman scattering. Raman tweezers allows us to fix and sterile manipulate with the trapped object and in the same time check the growth, viability, response to the external environment etc. by Raman signal evaluating. The investigations presented here include distinction of bacteria in general (staphylococcal cells), identification of bacteria strains (biofilm-positive and biofilm-negative) by using principal component analysis (PCA) and monitoring the influence of antibiotics.

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Silvie Bernatova, Ota Samek, Zdenek Pilat, Mojmir Sery, Jan Jezek, Petr Jakl, Martin Siler, Vladislav Krzyzanek, Pavel Zemanek, Veronika Hola, Milada Dvorackova, and Filip Ruzicka "Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291Y (8 May 2014); https://doi.org/10.1117/12.2052538

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KEYWORDS

Raman spectroscopy

Bacteria

Principal component analysis

Optical tweezers

Microorganisms

Visible radiation

Chemical analysis

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