Immobilization Of Unicellular Marine Organisms For Optical Characterization: A New Method

K Lofftus; A J Hunt; M S Quinby-Hunt; F Livolant; M F Maestre

doi:10.1117/12.945741

12 August 1988 Immobilization Of Unicellular Marine Organisms For Optical Characterization: A New Method

K Lofftus, A J Hunt, M S Quinby-Hunt, F Livolant, M F Maestre

Proceedings Volume 0925, Ocean Optics IX; (1988) https://doi.org/10.1117/12.945741
Event: 1988 Technical Symposium on Optics, Electro-Optics, and Sensors, 1988, Orlando, FL, United States

Abstract

A new method has been developed to study the light scattering properties of single, immobilized unicellular marine organisms. Cells of the dinoflagellate Prorocentrum micans were fixed1 and immobilized by mixing them into a solution that formed a transparent gel. The residual Rayleigh scattering caused by the gel was effectively eliminated by substituting a second liquid into the gel to match the index of refraction of the gel network, thereby producing a transparent support medium. Scattering measurements of the elements of the Mueller matrix were performed on a single Prorocentrum micans cell using a polarization-modulation scanning nephelometer.2 The angular dependence of Su, and normalized S12, S22, S33, S34, S13, and S14 are presented. The structure in all elements is very rich, cell orientation dependent, and completely reproducible. The measurements also revealed significantly non-zero values for all 16 elements of the scattering matrix and the non-equivalence of cross-diagonal elements. A striking feature of the measurements is the large peak values of S14 for a single immobilized cell. In recent years S14 has generally been reported to be zero for sea water and very small for laboratory systems. Measurements averaged over an ensemble of cells showed lower, but distinctly non-zero values in accord with earlier predictions.3

Citation Download Citation

K Lofftus, A J Hunt, M S Quinby-Hunt, F Livolant, and M F Maestre "Immobilization Of Unicellular Marine Organisms For Optical Characterization: A New Method", Proc. SPIE 0925, Ocean Optics IX, (12 August 1988); https://doi.org/10.1117/12.945741

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