Investigation of diseases through red blood cells' shape using photoacoustic response technique

Deblina Biswas; Abhijeet Gorey; Goerge C. K. Chen; Norman Sharma; Srivathsan Vasudevan

doi:10.1117/12.2079185

5 March 2015 Investigation of diseases through red blood cells' shape using photoacoustic response technique

Deblina Biswas, Abhijeet Gorey, Goerge C. K. Chen, Norman Sharma, Srivathsan Vasudevan

Proceedings Volume 9322, Dynamics and Fluctuations in Biomedical Photonics XII; 93220K (2015) https://doi.org/10.1117/12.2079185
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Photoacoustic (PA) imaging is a non-invasive real-time technique, widely applied to many biomedical imaging studies in the recent years. While most of these studies have been focussed on obtaining an image after reconstruction, various features of time domain signal (e.g. amplitude, width, rise and relaxation time) would provide very high sensitivity in detecting morphological changes in cells during a biological study. Different haematological disorders (e.g., sickle cell anaemia, thalassemia) exhibit significant morphological cellular changes. In this context, this study explores the possibility of utilizing the developed photoacoustic response technique to apply onto blood samples. Results of our preliminary study demonstrate that there is a significant change in signal amplitude due to change in concentration of the blood. Thus it shows the sensitivity of the developed photoacoustic technique towards red blood cell count (related to haematological disease like anaemia). Subsequently, morphological changes in RBC (i.e. swollen and shrunk compared to normal RBC) induced by hypotonic and hypertonic solutions respectively were also experimented. The result shows a distinct change in PA signal amplitude. This would serve as a diagnostic signature for many future studies on cellular morphological disorders.

Citation Download Citation

Deblina Biswas, Abhijeet Gorey, Goerge C. K. Chen, Norman Sharma, and Srivathsan Vasudevan "Investigation of diseases through red blood cells' shape using photoacoustic response technique", Proc. SPIE 9322, Dynamics and Fluctuations in Biomedical Photonics XII, 93220K (5 March 2015); https://doi.org/10.1117/12.2079185

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