Path-length stabilized low-coherent reflection-type quantitative phase microscope for nanometer-resolution profiling of plasma membrane

Toyohiko Yamauchi; Hidenao Iwai; Kentaro Goto; Shu Honma; Yutaka Yamashita

doi:10.1117/12.2077098

11 March 2015 Path-length stabilized low-coherent reflection-type quantitative phase microscope for nanometer-resolution profiling of plasma membrane

Toyohiko Yamauchi, Hidenao Iwai, Kentaro Goto, Shu Honma, Yutaka Yamashita

Author Affiliations +

Proceedings Volume 9336, Quantitative Phase Imaging; 933603 (2015) https://doi.org/10.1117/12.2077098
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We developed a portable and user-friendly prototype of low-coherent reflection-type quantitative phase microscope (QPM). Our setup is based on the full-field Linnik type phase-shifting interference microscope and is optimized for surface profiling of living cell’s membrane. Unlike commonly available transmission-type quantitative phase microscopes which reveal the optical thickness, our reflection-type setup can obtain the geometrical thickness (real shape) of the sample, decoupled with the refractive index. The coherence length of our imaging light source (halogen lamp) was approximately 1 micrometer so that we can selectively obtain the interference of the light reflected from the cell membranes whose reflectivity in culture medium is only on the order of 0.1%. Moreover our setup has a feedback controlled path-length stabilization circuit so that users can implement accurate phase shifting interferometry with one nanometer of reproducibility. The stabilization circuit allows installing our setup even in noisy environments such as biology labs without an optical bench. In this paper, we will also show our studies of recent biomedical applications, including imaging of cell plasma membrane and phase-resolved 3D tomography of living cells.

Citation Download Citation

Toyohiko Yamauchi, Hidenao Iwai, Kentaro Goto, Shu Honma, and Yutaka Yamashita "Path-length stabilized low-coherent reflection-type quantitative phase microscope for nanometer-resolution profiling of plasma membrane", Proc. SPIE 9336, Quantitative Phase Imaging, 933603 (11 March 2015); https://doi.org/10.1117/12.2077098

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